Author/Authors :
Eskandari, Khadijeh Nanobiotechnology Research Center - Baqiyatallah University of Medical Sciences, Tehran, Iran , Ghourchian, Hedayatollah Laboratory of Microanalysis - Institute of Biochemistry and Biophysics - University of Tehran, Tehran, Iran , Banaei, Aghdas Research Institute of Applied Science - Academic Center of Education - Culture and Research (ACECR) - Shahid Beheshti University, Tehran, Iran
Abstract :
A feasible and fast method for Adult Hemoglobin (A-Hb) and Fetal Hemoglobin (F-Hb) study was developed by immobilization of Hb on gold-coated magnetic iron oxide nanoparticles (GMNPs).The prepared GMNPs composite nanoparticles with
60 nm diameter were used as a carrier for the immobilization of Hb. The A-Hb and F-Hb were physically attached to the GMNPs nanoparticles. The direct electrochemistry of F-Hb and A-Hb showed a quasi-reversible cyclic voltammogram corresponding to the Heme group with a formal potential of 314 and -334 mV in 0.1M PBS (pH 6.2), respectively. The apparent charge transfer rate constant (ks) and transfer coefficient (α) for electron transfer between the electrode surface and protein were calculated as 0.29/s and 0.1 for F-Hb and 0.21/s and 0.47 for A-Hb. The linear concentration rangesare17.3–225 and 7.4-53 mM for F-H band A-Hb biosensors for H2O2detection. The lifetime of biosensor is more than 2 weeks.
Keywords :
Biosensor , Direct Electrochemistry , H2O2 , Gold-coated , Iron Oxide Nanoparticles , Hemoglobin
