Author/Authors :
Yousefi, Maryam Department - School of Public Health - International Campus - Tehran University of Medical Sciences, Tehran , Nejati, Ahmad Department - School of Public Health - Tehran University of Medical Sciences, Tehran , Zahraei, Mohsen Department - Center or Communicable Diseases Control - Ministry of Health and Medical Education, Tehran , Mahmoudi, Sussan Department - Center or Communicable Diseases Control - Ministry of Health and Medical Education, Tehran , Parhizgari, Najmeh Department - School of Public Health - Tehran University of Medical Sciences, Tehran , Jazayeri Farsani, Mohammad Department of Medical Microbiology - Center for Infection and Immunity Amsterdam Academic Medical Center - University of Amsterdam, Netherlands , Mahmoodi, Mahmood Department of Epidemiology and Biostatistics - School of Public Health - Tehran University of Medical Sciences, Tehran , Nategh, Rakhshandeh Department - School of Public Health - Tehran University of Medical Sciences, Tehran , Shahmahmoodi, Shohreh Department - School of Public Health - Tehran University of Medical Sciences, Tehran
Abstract :
Background and Objectives: Acute flaccid paralysis (AFP) is a complicated clinical syndrome with a wide range of potential
etiologies. Several infectious agents including different virus families have been isolated from AFP cases. In most
surveys, Non-polio Enteroviruses (NPEVs) have been detected as main infectious agents in AFP cases; however, there are
also some reports about Adenovirus isolation in these patients. In this study, NPEVs and Adenoviruses in stool specimens of
AFP cases with or without Residual Paralysis (RP) with negative results for poliovirus are investigated.
Materials and Methods: Nucleic acid extractions from 55 AFP cases were examined by nested PCR or semi-nested PCR
with specific primers to identify NPEVs or Adenoviruses, respectively. VP1 (for Enteroviruses) and hexon (for Adenoviruses)
gene amplification products were sequenced and compared with available sequences in the GenBank.
Results: From 55 fecal (37 RP+ and 18 RP-) specimens, 7 NPEVs (12.7%) (2 cases in RP+) and 7 Adenoviruses (12.7%) (4
cases in RP+) were identified. Echovirus types 3, 17 and 30, Coxsackie virus A8, and Enterovirus 80 were among NPEVs
and Adenoviruses type 2 and 41 were also identified.
Conclusion: Our finding shows that NPEVs and Adenoviruses may be isolated from the acute flaccid paralyses but there is
no association between the residual paralyses and virus detection.
Keywords :
Acute flaccid paralysis , Residual paralysis , Non-polio Enterovirus , Adenovirus