Title of article :
The Effect of Cationic Liposomes Encapsulating pcDNA3.1+PA Plasmids on Humoral Immune Response in Mice
Author/Authors :
Vahedi, Fatemeh Razi Vaccine and Serum Research Institute - Mashhad , Jaafari, Mahmoud Reza Department of Pharmacology - Mashhad University of Medical Science , Mahmoudi, Mahmoud Immunology Research Center - Mashhad University of Medical Science - Mashhad
Abstract :
Background: DNA vaccines are third generation vaccines which have made promises
to combat infectious diseases. Cationic liposomes are used as effective delivery systems
for DNA vaccines to generate stronger immunity. Objective: Encapsulation of
pcDNA3.1+PA plasmid, encoding protective antigen (PA) of Bacillus anthracis
(B. anthracis) into cationic liposomes, and evaluation of its effect on specific humoral
specific immunity against PA were aimed. Methods: The liposomes containing
pcDNA3.1+PA plasmids were prepared with phosphatidylcholine (PC), dioleoyl
phosphatidylethanolamine (DOPE) and 1,2-dioleoyl-3-trimethylammonium-propane
(DOTAP) using dehydration-rehydration method. BALB/c mice were immunized by
intramuscular (IM) injection to investigate the immunogenicity of the formulations. The
resulting specific antibodies against PA, total IgG, IgG1, IgG2a and IgG2b isotypes,
were evaluated by enzyme linked immunosorbent assay (ELISA) method.
Conclusion: A higher concentration of specific IgG against PA was found in sera of a
group immunized with the encapsulated plasmid compared with the naked plasmid
alone. This difference was significant for IgG1 isotype.
Keywords :
pcDNA3.1+PA , Mice , DNA Vaccine , DOPE- DOTAP , Cationic Liposome , Anthracis
Journal title :
Astroparticle Physics
