Title of article :
An Endogenous Immune Adjuvant Released by Necrotic Cells for Enhancement of DNA Vaccine Potency
Author/Authors :
Dorostkar, Rohollah Department of Virology - Faculty of Medical Sciences - Tarbiat Modares University - Tehran , Bamdad, Taravat Department of Virology - Faculty of Medical Sciences - Tarbiat Modares University - Tehran , Pouriayevali, Hassan Department of Virology - Faculty of Medical Sciences - Tarbiat Modares University - Tehran , Parsania, Masoud Department of Microbiology - Tehran Medical Branch - Islamic Azad University - Tehran
Abstract :
Background: Improving vaccine potency in the induction of a strong cell-mediated
cytotoxicity can enhance the efficacy of vaccines. Necrotic cells and the supernatant of
necrotic tumor cells are attractive adjuvants, on account of their ability to recruit
antigen-presenting cells to the site of antigen synthesis as well as its ability to stimulate
the maturation of dendritic cells. Objective: To evaluate the utility of supernatant of
necrotic tumor cells as a DNA vaccine adjuvant in a murine model. Method: The
supernatant of EL4 necrotic cells was co-administered with a DNA vaccine expressing
the glycoprotein B of Herpes simplex virus-1 as an antigen model under the control of
Cytomegalovirus promoter. C57BL/6 mice were vaccinated three times at two weeks
intervals with glycoprotein B DNA vaccine and supernatant of necrotic EL4 cells. Five
days after the last immunization, cell cytotoxicity, IFN-γ and IL-4 were evaluated.
Results: The obtained data showed that the production of IFN-γ from the splenocytes
after antigenic stimulation in the presence of the supernatant of necrotic EL4 cells was
significantly higher than the other groups (p<0.002). The flow cytometry results showed
a significant increase in the apoptosis/necrosis of EL4 cells in the mice immunized with
DNA vaccine and supernatant of necrotic EL4 cells comparing to the other groups
(p<0.001). Conclusion: The supernatant of necrotic cells contains adjuvant properties
that can be considered as a candidate for tumor vaccination.
Keywords :
Necrosis , Herpes Simplex Virus-1 , DNA Vaccine , Adjuvant
Journal title :
Astroparticle Physics