Author/Authors :
Hasheminia, Javad Cellular and Molecular Research Center - School of Medicine - Shahre Kord University of Medical Sciences - Shahre Kord , Zarkesh-Esfahani, Sayyed Hamid Department of Biology - School of Sciences - University of Isfahan , Tolouei, Sepideh Department of Parasitology and Mycolog- School of Medicine - Isfahan University of Medical Sciences , Shaygannejad, Vahid Department of Neurology - School of Medicine - Isfahan University of Medical Sciences - Isfahan , Shirzad, Hedaiatallah Department of Immunology - School of Medicine - Shahre Kord University of Medical Sciences , Chaleshtory, Morteza Hashemzadeh Cellular and Molecular Research Center - School of Medicine - Shahre Kord University of Medical Sciences - Shahre Kord
Abstract :
Background: Multiple sclerosis (MS) is a T cell mediated autoimmune disease with
unknown etiology. Appropriate MS therapeutic strategies need thorough understanding
of both disease etiology and pathogenesis mechanisms. Ligation of TLR-2 and TLR-4
stimulates the production of several cytokines leading to CNS autoimmunity and
neurodegenerative diseases. Objective: To find a relationship between MS disability
and TLR-2 and TLR-4 expression on mononuclear cells in the blood of MS patients.
Methods: Forty-five new case (NC) MS patients (33 females and 12 males) and 45 age
and gender-matched healthy controls (HC) were recruited to the study. PBMCs were
prepared and the expressions of TLR-2 and TLR-4 were assessed by flowcytometry
technique using appropriate monoclonal antibodies. Results: Our results showed that
the expression of TLR-2 and TLR-4 proteins in the patients group was significantly
higher than that of healthy controls. TLR-2 but not TLR-4 was correlated with expanded
disability status scale (EDSS) scores. Conclusion: High expressions of TLR-2 and
TLR-4 may represent a state of innate immune activation in patients with MS.
Keywords :
Multiple Sclerosis , PBMC , TLR-4 , TLR-2
