Author/Authors :
Soheili, Masoud School of Allied Medical Sciences - Shahid Beheshti University of Medical Sciences, Tehran , Arefi Oskouie, Afsaneh Department of Basic Sciences - School of Allied Medical Sciences - Shahid Beheshti University of Medical Sciences, Tehran , Rezaei Tavirani, Mostafa School of Allied Medical Sciences - Shahid Beheshti University of Medical Sciences, Tehran , Aliakbarzadeh, Ghazaleh Department of Chemistry - Faculty of Science - University of Tehran, Tehran , Salami, Mahmoud Kashan University of Medical Sciences, Kashan
Abstract :
Introduction: Metabolites are the intermediate and natural low molecular weight products of metabolic reactions which naturally occur within cells. Metabolomics is a post-genomics study that analyzes metabolic profile in all biological samples. Gas chromatography-mass spectrometry (GC-MS) is a powerful technique that detects volatile components. This study evaluates the metabolite profile of serum with a simple method for derivatization. Methods: In this study, the metabolic profile of serum sample of 16 rats was analyzed using GC-MS after protein precipitation, using acetonitrile, and derivatize with a method of less in number and volume of materials. Acquisition chromatograms were pre-processed and analyzed using National Institute of Standards and Technology (NIST) library.Results: 35 metabolites with probability of >60% perfect match were detected according to spectrum fragments analysis by NIST library.
Conclusion: This study represents the metabolome profile of serum instead of the change of metabolites that can be evaluated with a simple derivatization method.
Keywords :
metabolomics , rat , serum , GC-MS
