Author/Authors :
Nourinia, Ramin Ophthalmic Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ahmadieh, Hamid Ophthalmic Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Rezaei Kanavi, Mozhgan Ophthalmic Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Soheili, Zahra-Soheila National Institute of Genetic Engineering and Biotechnology, Tehran, Iran , Akrami, Hassan Department of Biology - Faculty of Science - Razi University, Kermanshah, Iran , Samiei, Shahram Iranian Blood Transfusion Organization, Tehran, Iran
Abstract :
Purpose: To evaluate the effect of placental growth factor (PlGF) gene knockdown in
a murine model of laser-induced choroidal neovascularization.
Methods: Choroidal neovascularization was induced in the left eyes of 11 mice by
infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresponding to
PlGF mRNA was administered intravitreally by Hamilton syringe in all subjects. One
month later, fluorescein angiography and histolologic examination were performed.
Results: No leakage was apparent in the 11 eyes treated with siRNA cognate to PlGF.
The results of histological evaluation were consistent with angiographic findings showing absence of choroidal neovascularization.
Conclusion: Knockdown of the PlGF gene can inhibit the growth of laser-induced
choroidal neovascularization in mice.
