Title of article :
High Quality RNA Isolation from Leaf, Shell, Root Tissues and Callus of Hazelnut (Corylus avellana L.)
Author/Authors :
Khosravi, Hossein Department of Plant Biotechnology - faculty of Agriculture - Bu-Ali Sina University, Hamedan, Iran , Deljou, Ali Department of Plant Biotechnology - faculty of Agriculture - Bu-Ali Sina University, Hamedan, Iran , Rashidi Monfared, Sajad Department of Biotechnology - faculty of Agriculture - Tarbiat Modarres University, Tehran, Iran
Pages :
7
From page :
89
To page :
95
Abstract :
Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one of the most im-portant nuts plants in the world. The presence of the taxol and other taxanes in hazelnut plant necessitates explain-ing their biosynthesis pathway and identifying the candidate genes. Therefore, an easy and practical method is nec-essary for RNA extraction from hazelnuts. Hazelnut has high levels of phenolic compounds. High amounts of poly-phenolic and polysaccharide compounds in plants could be causing problems in RNA extraction procedures. To avoid these problems, a simple and efficient method can be used based on cetyltrimethylammonium bromide (CTAB) extraction buffer and lithium chloride for extraction of high quality RNA from different parts of hazelnut plant. Using this method, a high-quality RNA sample (light absorbed in the A260/A280 was 2.04
Keywords :
Taxol , RNA extraction , Hazelnut , CTAB extraction
Journal title :
Astroparticle Physics
Serial Year :
2017
Record number :
2433507
Link To Document :
