Author/Authors :
Mashinchian Moradi, A. Department of Marine Biology - Graduate School of Marine Science and Technology - Science and Research Branch, Islamic Azad University, Tehran, Iran , Mozdarani, H. Department of Medical Genetics - Faculty of Medical Sciences - Tarbiat Modares University, Tehran, Iran , Alidoust Salimi, P. Department of Marine Biology - Graduate School of Marine Science and Technology - Science and Research Branch, Islamic Azad University, Tehran, Iran , Alidoust Salimi, M. Department of Marine Biology - Graduate School of Marine Science and Technology - Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract :
Malathion (S-(1, 2-dicarboethoxyethyl) O, O-dimethyl phosphorodithioate) is one of the organophosphate
pesticides comprehensively used in agriculture fields throughout the world. In spite of widely used, little information are
available about DNA damage in aquatic organisms. Therefore, the present study carried out to investigation of DNA damage
induced by malathion in Cyprinus carpio (Pisces: Cyprinidae) using single cell gel electrophoresis. The condition of experiment
was determined in static system. The specimens were exposed to different non-lethal concentrations (0.5, 1.5 and 3 mgL-1) of the
malathion for 96 hour (short-time exposed). Our results showed, the specimens exposed to different concentrations of malathion
exhibited significantly higher DNA damage in their blood cells than the control sample (P<0.05). This study confirmed that the
comet assay is useful method in determining genotoxicity of pesticides. Fish can be used for biomonitoring of the genotoxic
pollutants in aquatic environment. Furthermore, DNA strand breakage can be used as biomarker in ecotoxicological studies.
Keywords :
Comet assay , Insecticides , Genotoxicity , Genetic Damage Index (GDI) , Fish
