Author/Authors :
Savar Sofla, S. Animal Science Research Institute of Iran (ASRI) - Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran , Seyedabadi, H.R. Animal Science Research Institute of Iran (ASRI) - Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran , Javanrouh Aliabad1and, A. Animal Science Research Institute of Iran (ASRI) - Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran , Seyed Sharifi, R. Department of Animal Science - Faculty of Agricultural and Natural Resources - University of Mohaghegh Ardabili, Ardabil, Iran
Abstract :
Phylogenetic relationships and genetic variation between two Iranian sheep breeds were analyzed using cytochrome b (cyt-b) gene sequences. The genomic DNA was isolated by salting out method and amplified cytochrome b gene using polymerase chain reaction restriction (PCR) method with a pair of primer. A par-tial sequence of cyt-b gene of Iranian sheep is 780 bp and contained 13 variable sites and 11 haplotypes. Phylogenetic analysis of haplotype in the combination with the sheep from GenBank showed that Iranian sheep made a separated cluster. This study is provided useful information for understanding relationships between breeds from different parts of the world. This study may simplify the future researchers and breed-ers for better understanding the genetic structure and breed differentiation for designing future breeding strategies to the conservation of animal genetic resources.
Keywords :
phylogenetic analysis , mitochondrial cytochrome b gene , Iranian sheep , genetic diversity
