Author/Authors :
Pessaran, Zohreh Department of Immunology - Isfahan University of Medical Sciences, Isfahan , SahebFosul, Freshteh Department of Immunology - Isfahan University of Medical Sciences, Isfahan , Oreizi, Farzad Department of Immunology - Isfahan University of Medical Sciences, Isfahan , Ghavaminejad, Ahmad Department of Immunology - Isfahan University of Medical Sciences, Isfahan , Kiani, Abolfazl Department of Immunology - Isfahan University of Medical Sciences, Isfahan , Dana Siadat, Zahra Department of Immunology - Isfahan University of Medical Sciences, Isfahan
Abstract :
Tuberculosis is a chronic mycobacterial infection. The main effector cells against
mycobacterium tuberculosis are CD4+ T lymphocytes. Our objective in this research
was to evaluate the quantity of T lymphocytes and their subpopulations before and
after treatments with combination of 4 drugs (Rifampcin, Isoniaside, pyrasinamide,
Ethambutal) for 2 months directly in sputum-positive tuberculosis patients. Twenty
patients as cases and twenty healthy people were selected as controls. Flow cytometry
was used for TCD3+, TCD4+ and TCD8+ lymphocytes by using monoclonal
antibodies. Our results indicated that there was alteration in cell mediated immunity
during tuberculosis showing itself as decrease in TCD3+ and TCD4+ lymphocytes and
increase in TCD8+ lymphocytes. The changes in TCD3+ and TCD4+ but not in
TCD8+ were reversible after 2 months of treatment.
Keywords :
Cell Mediated Immunity , Immunophenotyping , T Lymphocytes , Tuberculosis
