Title of article :
The Effect of Vitamin E on Splenocytes Apoptosis of Gamma-Irradiated BALB/c Mice
Author/Authors :
Maroufi, Bahman University of Tehran, Tehran , Ardestani, Kaboudanian Sussan University of Tehran, Tehran , Kariminia, Amina Department of Immunology - Pasteur Institute of Iran, Tehran , Naderimanesh, Hussain Biophysics Department - Faculty of Science - Tarbiat Modaress University, Tehran
Abstract :
Apoptosis, a physiologic mechanism to eliminate unwanted cells, is also induced by
ionizing irradiation, through production of free radicals. It has been demonstrated that
antioxidants such as vitamin E are able to protect cells from damage caused by free
radicals. Taken together we found it reasonable to make an attempt to evaluate the
protective effect of vitamin E against apoptosis. The irradiated mice received 1 Gy/day
gamma radiation for one day (low dose) or for three successive days (high dose, 3Gy).
The splenocytes were then isolated at 6, 14 and 24 h after exposure. DNA gel
electrophoresis and DNA fragmentation assay were done in addition to the evaluation
of splenocytes cytology.
Our results showed that Vitamin E can reduce apoptosis against low dose
irradiation. However it is not able to completely block programmed cell death in high
dose irradition
Keywords :
Apoptosis , Free Radicals , Gamma Radiation , Vitamin E
Journal title :
Astroparticle Physics
