Author/Authors :
Fang, Wen-Liang Department of Immunology - West China School of Preclinical and Forensic Medicine - Sichuan University - Chengdu - Sichuan, China , Gao, Lin-Bo Department of Forensic Biology - West China School of Preclinical and Forensic Medicine - Sichuan University - Chengdu - Sichuan, China , Liang, Wei-Bo Department of Forensic Biology - West China School of Preclinical and Forensic Medicine - Sichuan University - Chengdu - Sichuan, China , Xue, Hui Department of Immunology - West China School of Preclinical and Forensic Medicine - Sichuan University - Chengdu - Sichuan, China , Bai, Peng Department of Forensic Biology - West China School of Preclinical and Forensic Medicine - Sichuan University - Chengdu - Sichuan, China , Lv, Mei-Li Department of Immunology - West China School of Preclinical and Forensic Medicine - Sichuan University - Chengdu - Sichuan, China , Wang, Yan-Yun Department of Immunology - West China School of Preclinical and Forensic Medicine - Sichuan University - Chengdu - Sichuan, China , Zhou, Bin West China Second University Hospital - Sichuan University - Chengdu - Sichuan, China , Zhang, Lin Department of Immunology - West China School of Preclinical and Forensic Medicine - Sichuan University - Chengdu - Sichuan, China
Abstract :
Several asthma susceptibility loci, including a region containing the vitamin D receptor
(VDR) gene located at chromosome 12q, have been identified using genome-wide screens.
Our aim is to investigate the association between single nucleotide polymorphisms (SNPs) in
VDR gene and asthma.
One hundred one asthma patients and 206 healthy controls were enrolled in this study.
Genotypes were determined using a polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP) strategy and DNA sequencing.
The results showed that there was no significant differences in the genotype and allele
frequencies of Fok I and Bsm I polymorphisms between asthma patients and the controls in
the Chinese Hans (For Fok I: or = 1.15, 95% CI: 0.82–1.60; for Bsm I: or = 1.44, 95% CI:
0.87–2.38).
It is suggested that Fok I and Bsm I polymorphisms of VDR gene may not significantly
contribute to the development of asthma in the Chinese Hans.
Keywords :
Asthma , Chinese , DNA Sequencing , Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) , Single nucleotide Polymorphism (SNP) , Vitamin D receptor (VDR)
