Author/Authors :
Pasandideh, R. Department of Animal Science - Faculty of Animal and Food Science - Khuzestan Agricultural Science and Natural Resources University, Ahvaz, Iran , SeyfiAbad Shapouri, M.R. Department of Pathobiology - Faculty of Veterinary Medicine - Shahid Chamran University of Ahvaz, Ahvaz, Iran , Beigi Nassiri, M.T. Department of Animal Science - Faculty of Animal and Food Science - Khuzestan Agricultural Science and Natural Resources University, Ahvaz, Iran
Abstract :
Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. There-fore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for se-rological identification bovine ephemeral fever (BEF)-infected animals. The aim of this study was to pro-duce a monoclonal antibody (MAb) against recombinant G1 antigen expressed into Escherichia coli. For this purpose, somatic cell hybrids between SP2/0 myeloma cells and spleen cells derived from Balb/c mice immunized with maltose-binding protein (MBP)-G1 fusion protein were established. After three rounds of cloning, the stability of antibody secretion in the positive clones was confirmed by ELISA and the reactivity of the MAbs against recombinant G1 was verified by Western blot analysis. The specific MAbs produced against recombinant G1 antigen in this study could be used for establishing BEFV diagnostic experiments in the future.
Keywords :
recombinant G1 protein , monoclonal antibody , bovine ephemeral fever virus , hybridoma
