Association of FGFR2 and TOX3 Genetic Variants With the Risk of Breast Cancer in Iranian Women

Background: Breast cancer is the most common cause of cancer-related death in women worldwide. Novel genetic markers for breast cancer susceptibility have been identified in population-based studies. The aim of this study was to examine the association of two single-nucleotide polymorphisms (SNPs) of FGFR2 (rs1219648) and TOX3 (rs8051542) with the risk of breast cancer in Iranian women. Methods: Breast cancer patients (n = 126) and healthy controls (n = 160) were genotyped for SNPs in FGFR2 (rs1219648) and TOX3 (rs8051542) using the tetraprimer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). Also, immunohistochemical tests for human epidermal growth factor receptor-2, estrogen receptor, and progesterone receptor were carried out on breast tumor tissues. Results: TOX3 (rs8051542) CC (OR = 1.24; 95% CI, 0.72-0.214; P < 0.001) and FGFR2 (rs1219648) GG (OR = 62.0; 95% CI, 23.63-162.66; χ2 =132.775 ; P < 0.001) polymorphism was significantly associated with breast cancer. The association was also significant between breast cancer risk and TOX3 (rs8051542) TC and FGFR2 (rs1219648) AG variants. Conclusion: Our findings suggested that genetic variants of FGFR2 (rs1219648 AG) and TOX3 (rs8051542 TC) can be potential candidate biomarkers for breast cancer risk.