Author/Authors :
Asghari, Ali Tabriz University of Medical Sciences, Tabriz , Montaseri, Azadeh Department of Anatomical Sciences - Faculty of Medicine - Tabriz University of Medical Sciences, Tabriz , Khaki, Amir Afshin Alzahra Hospital - Tabriz University of Medical Sciences, Tabriz
Abstract :
Objective: Everyday use of different types of electrical instruments and appliances has caused a large
number of people to constantly be under the influence of electromagnetic fields.
Materials and Methods: For the purpose of this study, 40 female rats were randomly chosen from among
3 months old rats from the animals’ laboratory and they weighed 20 + 200 g. Then, they were randomly
divided into 4 groups; control (n = 10), experiment 1 (Ex1) (n = 10), experiment 2 (Ex2) (n = 10), and
experiment 3 (Ex3) (n = 10). During the experiment, all 4 groups were maintained in the same conditions
and received the same feeding. The experiment groups 1, 2, and 3 were under the influence of a 50 Hz
electromagnetic field (EMF) for 8 weeks. Subsequently, the second and third groups were kept away from
the EMF effect for another 8 weeks. At the end of the study, after removal of the ovaries by
glutaraldehyde, they were prepared for examination using an electron microscope. Group Ex2 rats were
not sacrificed and were maintained in the normal laboratory environment for another 8 weeks away from
the impacts of EMF. The rats were fed vitamin E(100 mg/kg) and fennel extract (1.5 g per body weight)
every day orally and at the end of the second 8 weeks samples were taken. During the second 8 weeks,
group Ex3 was kept in normal conditions without the use of vitamin E and fed fennel extract, and then,
samples were taken. Samples were taken simultaneously from 10 rats of the control group and Ex1 group.
Results: The results from the mitochondria in the ovary in the groups under the influence of
electromagnetic waves indicated that this intracellular organ, compared to samples from the control
group, was deformed and the majority of the organs were vacuolated. The mitochondrial vacuolization of
the first to fourth groups were 1 ± 0.55, 9 ± 0.55, 6 ± 0.55, and 11 ± 0.55, respectively.
Conclusion: Vitamin E and fennel extract can reduce the damaging effects of non-ionizing radiation with
50 Hz frequency on the ovarian follicles
Keywords :
Electromagnetic Field (EMF) , Fennel , Mitochondria , Ovary
