Author/Authors :
Norollahi, Amin Department of Bioloy - Damghan Branch - Islamic Azad University, Damghan , Kokhaee, Parviz Faculty of Medicine - Department of Immunology - Semnan University of Medical Sciences, Semnan , Rashidy-Pour, Ali Faculty of Medicine - Research Center of Physiology - Semnan University of Medical Sciences, Semnan , Hojati, Vida Department of Bioloy - Damghan Branch - Islamic Azad University, Damghan , Norollahi, Elham Faculty of Medicine - Research Center of Physiology - Semnan University of Medical Sciences, Semnan , Vahedi Larijani, Laleh Department of Pathology - BoAli Sina Hospital - Mazandaran University of Medical Sciences, Sari , Samadani, Ali Akbar Faculty of Medicine - Research Center of Physiology - Semnan University of Medical Sciences, Semnan
Abstract :
Objective: Optimal quality and quantity of extracted RNA is the first step in molecular biology analysis and investigation. In this way, several methods have been proposed in order to obtain the best quality of RNA in different cases. On the other hand, RNA extraction from cells and tissues is different.
Materials and Methods: In this study, the effects of 4 common RNA extraction kits including Trizol, AccuZol, Ribozol and TriPure and also the effect of RNAlater and liquid nitrogen were compared and studied on 50 breast cancer and 50 gastric cancer tissues. Remarkably, the quality of the extracted RNA was investigated using real-time PCR TaqMan assay on HER2 gene.
Results: The results showed better relative quality of extracted RNA with Trizol kit compared to other kits in this study.
Conclusion: Conspicuously, fewer amount of tissues between 10 to 30 mg lead to gain a much better quality of RNA. Meanwhile, the expression of HER2 gene indicates a suitable performance of extracted RNA qualitatively and quantitatively. Notably, GAPDH gene was used as internal control in all samples.
Keywords :
RNA extraction , Histopathological , Gastric cancer , Breast cancer
