Author/Authors :
Ghoorchibeigi, Mona Department of Chemistry - Science and Research Branch, Islamic Azad University, Tehran, Iran , Larijani, Kambiz Department of Chemistry - Science and Research Branch, Islamic Azad University, Tehran, Iran , Aberoomand Azara, Parviz Department of Chemistry - Science and Research Branch, Islamic Azad University, Tehran, Iran , Zare, Karim Department of Chemistry - Science and Research Branch, Islamic Azad University, Tehran, Iran , Mehregan, Iraj Department of Biology - Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract :
The water distillated essential oil of Citrus medicacollected from Ramsar, Province of Mazandaran, North of Iran collected in December 2013, was analyzed using gas chromatography (GC) and gas chromatography-mass spectroscopy (GC-MS). The yield of oil was 0.20% w/w. Sixtheen components representing 99.6% of the essential oil were characterized. Limonene (46.9%) and γ-terpinene (34.3%) were identified as the main constituents in the volatile oil. The antioxidant ability of the oil was examined by free radical scavenging method using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical at different concentration of the oil. TheCitrus medica oil exhibited free-radical-scavenging properties with IC50 value of 348.72μg ml-1.
Keywords :
Citrus medica , Essential oil , Radical Scavenging , DPPH