Isolation and identification of Bacillus subtilis producing biosurfactant and study the optimum condition of biosurfactant production

102 bacterial isolates were obtained from 40 oil contaminated soil samples from various automobile workshops, cars fuel stations, oil refineries of five different areasinIraq. All these isolates were subjected , to molecular identification based on the 16S rDNA by two sets of primers (B16SF and B16SR),(27Fand1492R),from these isolates 51 of the identified isolates belonged to Bacillus subtilis.Maximumbiosurfactant production from these isolates was found in 8 isolates symbol (S1,S2,S3,S4,S5,S6,S7,S8) , it was achieved by using olive oil,ammonium sulfate,at pH 7.0 ,30ºC and incubation period for 72 hrs.