Title of article :
A novel two-step method for synthesis, purification and characterization of mebrofenin in Pars Isotope Company
Author/Authors :
Nabati, Mehdi Synthesis and Molecular Simulation Laboratory - Chemistry Department - Pars Isotope Company, Tehran, Iran , Maghsoudloo-Mahalli, Ahmad Synthesis and Molecular Simulation Laboratory - Chemistry Department - Pars Isotope Company, Tehran, Iran , Mohammadnejad-Mehrabani, Hossein Synthesis and Molecular Simulation Laboratory - Chemistry Department - Pars Isotope Company, Tehran, Iran , Movahed-Tazehkand, Hossein Synthesis and Molecular Simulation Laboratory - Chemistry Department - Pars Isotope Company, Tehran, Iran
Abstract :
This article presents the novel method for synthesis of the N-[2,4,6-trimethyl-3-bromoacetanilid]iminodiacetic acid (bromo-HIDA, or Br-HIDA) compound in two steps. The Technetium-99m-labeled mebrofenin, transported in blood by binding to albumin in the same manner as the indocyanine green (ICG) clearance test, can be used for hepatobiliary scintigraphy in the assessment of liver function. The important goal of this research work was to synthesize and purify the mebrofenin molecule by our novel method. Then, its purification was identified by NMR, HPLC and FT-IR analyses and compared with standard obtained bromo-HIDA. The comparison between the HPLC analyses of our synthesized mebrofenin and obtained compound shows high accuracy of our method.
Keywords :
Br-HIDA , FT-IR spectroscopy , HPLC technique , Mebrofenin , Purification , Radiopharmaceutical
Journal title :
Astroparticle Physics
