Title of article :
A study on the assessment of DNA strand-breaking activity by silver and silica nanoparticles
Author/Authors :
Vahdati, Ali R Islamic Azad University, Tonekabon , Sadeghi, Babak Department of Chemistry - Tonekabon Branch - Islamic Azad University Tonekabon, Tonekabon
Abstract :
We measured the DNA strand-breaking activity of nanoparticle by agarose gel electrophoresis. Plasmid DNA was
mixed with nanosilver and nano-silica powders in an aqueous solution. Then the mixture was applied to 1% of
agarose gel electrophoresis at 75 V for 30 min. We used 300 nm of UV irradiation in order to visualize the DNA
bands. The DNA strand-breaking activity of nanomaterial was measured through measuring the reduction of
super-coiled DNA to circular form of DNA.
Keywords :
Silver nanoparticles , DNA , Nanotechnology
Journal title :
Astroparticle Physics
