Title of article :
Effect of exogenous proline on protein pattern changes in Citrus sinensis (L.) Osbeck under in vitro salt stress
Author/Authors :
Abbas, Muayed F. College of Agriculture - Basrah University, Basrah, Iraq. , Jasim, Abbas M. College of Agriculture - Basrah University, Basrah, Iraq. , Al-Taha, Huda A. College of Agriculture - Basrah University, Basrah, Iraq
Abstract :
Effect of exogenous proline treatment on growth and protein pattern changes of plantlets of
Citrus sinensis (L.) Osbeck cv. Local orange under NaCl stress was investigated. Under salt stress
conditions, the height and leaf number of the plantlets decreased significantly, whereas the addition of
proline to the culture media significantly alleviated the inhibitory effect of salinity. SDS-PAGE analyses of
extracted protein revealed that plantlets grown under NaCl stress (10, 40 and 50mM) showed the
presence of a high molecular weight protein (82.7, 81.3 and 81.5 kDa). The addition of proline at 50 and
75mg l-1 to the culture media induced the synthesis of three new proteins (20.4-21.6, 40.8-41.4 and
69.7-70.2 kDa). It is concluded that exogenous proline treatment induced the synthesis of salt
responsive proteins, in addition to its well known physiological roles.
Keywords :
Citrus sinensis (L.) , Proline , SDS-PAGE , salt stress
Journal title :
Astroparticle Physics
