Plantlet regeneration from root segments of Date palm tree (Phoenix dactylifera L. cv. Barhee) producing by in vitro culture

This study was conducted during two seasons growth (2010/2011) in order to induction of root segments taken from plantlets producing by in vitro culture on growth and differentiation to produce direct and indirect shoots by organogenesis method. The results showed the ability of root segments to produce direct shoots when cultured by MS medium supplied with 2.0 mg.l-1 BA and 0.2 mg.l-1 NAA. This treatment was a high significant in the percentage of response for direct shoot induction and number of shoots/explant comparison with other treatments reached 41.67 % and 4.6, respectively. Also, these results showed the possibility of root segments on callus induction when cultured in MS medium supplemented with 40 mg.l-1 2,4-D and 3 mg.l-1 2ip. This callus formation gave a high significant rate of response to producing the indirect shoots when this callus is re-cultured at the first time in MS medium supplied with 0.5 mg.l-1 BA and 0.1 mg.l-1 NAA, which reached to 53.34 %. Also, the MS medium supplied with the same concentration of BA recorded the highest rate of proliferation reached 5.2 shoot/100 mg callus.