UV-Visible spectrophotometry of Repaglinide in bulk and in formulation by using methyl orange as reagents

Two sensitive spectrophotometric methods are presented for the assay of Repaglinide in bulk drug and in formulations using methyl orange, as reagents. Oxidant by reacting with a fixed amount of either methyl orange and measuring the absorbance at 485.2 nm (method-I) and measuring the absorbance at 618.9 nm (method-II). Developed method is based on the formation of extractable colored complex of drug with coloring agent Methyl orange dye. A wavelength maximum was found to be 618.9 nm. The concentration range of 15-50 μg ml-1 with linear regression of 0.9995, while the percentage recovery, LOD and LOQ were 99.42-99.08 %, 2.17 μg ml-1 and 1.08 μg ml-1 respectively. The result of analysis have been validated statistically and also by recovery studies. From the percentage recovery and specificity studies it was concluded that there was no interference of common additives during the estimation. This proves the suitability of this method for the routine quality control analysis of the Repaglinide in formulation.