Effect of some growth regulators on proliferation of myrtle (Myrtus communis L. cv. Local) plant by in vitro culture

The study was carried out at Plant Tissue Culture, Marine Sciences Center, University of Basra, Iraq. The aim of this study is micro propagation of myrtle plant by shoot multiplication technique. The results showed that shoots were formed on MS medium supplemented with 1.0 mg.L-1 BA and 1.0 mg.L-1 kinetin after ten weeks from culture (7.0 shoots). Also, that shoot length and number of leaves/shoot were reached 4.5 cm and 8.20 leaves/shoot, respectively after ten weeks from culture. As well as the MS medium supplemented with 5.0 mg.L-1 NAA and 5.0 mg.L-1 IBA gave the highest rooting ratio (100%). Acclimatization was successful for 100% of the rooted plants. The results showed that plant height and number of leaves per shoot were increased to 6.67 cm and 10.00 leaves/shoot, respectively after two weeks from plant acclimatization. Then, shoots increased with increasing age of plant and reached the highest values after six weeks from acclimatization (10.33 cm and 13.20 leaves/shoot, respectively).