Title of article :
In vitro antitumor activity of patulin on cervical and colorectal cancer cell lines
Author/Authors :
Abastabar, M. Mazandaran University of Medical Sciences , Akbari, A. Iran University of Medical Sciences , Akhtari, J. Mazandaran University of Medical Sciences - Department of Medical Nanotechnology , Hedayati, M.T. Mazandaran University of Medical Sciences , Shokohi, T. Mazandaran University of Medical Sciences , Mehrad-Majd, H. Mashhad University of Medical Sciences , Ghalehnoei, H. Mazandaran University of Medical Sciences , Ghasemi, S. Mazandaran University of Medical Sciences
Abstract :
Background and Purpose: Patulin is a mycotoxin produced by some molds, especially Aspergillus and Penicilium, and is responsible for mycotoxicosis in animals and humans.
There is still not very detailed data about the anti-cancer potency of patulin, but some reports demonstrated that it induces cellular apoptosis and toxicity.
Materials and Methods: To determine the efficacy of patulin as a therapeutic strategy for cervical and colorectal cancers, we investigated its effects on HeLa, SW-48, and MRC-5 cell lines. Cell lines were exposed to various concentrations of patulin (i.e., 0.5, 1, 2, and 4 μM), then using methyl thiazolyl tetrazolium (MTT) and bromo-2′-deoxyuridine (BrdU) assays, the rates of apoptosis and cell viability were determined.
Results: The obtained results showed a significant reduction in cell viability and apoptosis induction in a dose-dependent manner. Among all the cell lines, the highest growth inhibition rate was obtained at the 4 μM concentration of patulin.
Conclusion: Our results suggested that patulin could significantly decrease tumor growth in human cervical and colorectal cancer models
Keywords :
Cancer , HeLa cells , Mycotoxin , Patulin
Journal title :
Astroparticle Physics
