Author/Authors :
Shahini Shams Abadi, Milad Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran , Siadat, davar Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran , vaziri, farzam Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran , davari, farzam Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran , davari, mehdi Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran , fateh, abolfazl Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran , Pourazar, shahin Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran , Abdolrahimi, farid Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran , Ghazanfari, morteza Department of Mycobacteriology and Pulmonary Research - Pasteur Institute of Iran, Tehran
Abstract :
Background:
The pathogenesis of nontypeable Haemophilus influenzae (NTHi) begins with adhesion to the rhinopharyngeal mucosa. Almost 38–80% of NTHi clinical isolates produce proteins that belong to the High Molecular Weight (HMW) family of adhesins, which are believed to facilitate colonization.
Methods:
In the present study, the prevalence of hmwA, which encodes the HMW adhesin, was determined for a collection of 32 NTHi isolates. Restriction Fragment Length Polymorphism (RFLP) was performed to advance our understanding of hmwA binding sequence diversity.
Results:
The results demonstrated that hmwA was detected in 61% of NTHi isolates. According to RFLP, isolates were divided into three groups.
Conclusion:
Based on these observations, it is hypothesized that some strains of nontypeable Haemophilus influenzae infect some specific areas more than other parts.
