Author/Authors :
Gharavi, MJ Dept. of Medical Parasitology - Tehran University of Medical Sciences, Tehran , Nobakht, M Dept. of Histology and Neuroscience - School of Medicine - Tehran University of Medical Sciences, Tehran , Khademvatan, S Dept. of Parasitology - Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , Fani, F Dept. of Pharmacology - Islamic Azad University, Tehran, Iran , Bakhshayesh, M Cellular and Molecular Research Center - Tehran University of Medical Sciences, Tehran , Roozbehani, M Faculty of Allied Medicine - Tehran University of Medical Sciences, Tehran
Abstract :
Background: The aim of the present study was to investigate the immunomodulation effects of aqueous garlic extract
(AGE) in the cultured macrophages infected by Leishmania major.
Methods: After J774 macrophages proliferation in RPMI1640 and incubation with Leishmania for 72 hours, AGE was
added in doses of 9.25, 18.5, 37, 74 and 148 mg/ml for 18, 24 and 48 hours and cell culture supernatants were harvested.
The Leishmania infected J774 cells to assess the cell viability was examined using trypan blue and methylthiazol
tetrazolium assay (MTT). An enzyme-linked immunosorbent assay (ELISA) was performed on cell culture supernatants for
measurement of interleukin IL-10 and IL-12.
Results: Dose of 37 mg/ml for 48 hours of garlic extract was the most potent dose for activation of amastigotes infected
macrophages. In addition, AGE increased the level of IL-12 in Leishmania infected cell lines significantly.
Conclusions: AGE treated cell is effective against parasitic pathogens, and AGE induced IL-12 differentially affected the
immune response to invading Leishmania parasites.
Keywords :
Leishmania major , Aqueous garlic extract , IL-10 , IL-12 , Macrophage , Iran
