Author/Authors :
Zaini, F Dept. of Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , Kanani, A Dept. of Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , Fateh, R Dept. of Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , Farahyar, Sh Dept. of Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , Nazeri, M Dept. of Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran , Falahati, M Dept. of Parasitology and Mycology - School of Medicine - Tehran University of Medical Sciences, Tehran , Salimi-Asl, M Dept. Of R&D - Behmedico Company, Tehran , Saemi, N Dept. of Nutrition - Allied Medical School - Tehran University of Medical Sciences, Tehran , Kargar Kheirabad, A Hormozgan Research Centre for Infectious and Tropical Disease - Hormozgan University of Medical Sciences, Hormozgan
Abstract :
Background: The aim of this study was identification of the epidemiology of Prototheca zopfii species from the milk
samples of dairy cattle in Isfahan, central Iran.
Methods: Milk samples were obtained from 230 dairy cattle, 130 with and 100 without mastitis, in Isfahan. The
samples were cultured in Prototheca Isolation Medium (PIM) and Sabouraud’s dextrose agar. All P. zopfii isolates were
identified by morphological and biochemical methods. Then, as a confirmatory test they were examined by genotypespecific
PCR.
Results: Four P. zopfii strains (3.07%) were isolated from the 130 samples of dairy cattle with clinical mastitis and
there was no isolation from totally 100 samples of healthy bovines without mastitis. Specific PCR product (about 946
bp) was detected in four isolates.
Conclusion: It seems that P. zopfii genotype II plays a key role in affecting bovine mastitis that confirmed other
previous studies. Our study was the first, which identified the Prototheca species by traditional and molecular methods
in Iran and Middle East as well.
