Author/Authors :
Vossughinia, H Dept. of Gastroenterology - Ghaem Hospital - Mashhad University of Medical Sciences, Mashhad, , Goshayeshi, LA Dept. of Gastroenterology - Ghaem Hospital - Mashhad University of Medical Sciences, Mashhad , Sima, H Dept. of Gastroenterology - Ghaem Hospital - Mashhad University of Medical Sciences, Mashhad , Kazemi, A Dept. of Gastroenterology - Ghaem Hospital - Mashhad University of Medical Sciences, Mashhad , Erfani, S Dept. of Gastroenterology - Ghaem Hospital - Mashhad University of Medical Sciences, Mashhad , Abedini, S Dept. of Gastroenterology - Ghaem Hospital - Mashhad University of Medical Sciences, Mashhad Dept. of Gastroenterology - Ghaem Hospital - Mashhad University of Medical Sciences, Mashhad , Rafatpanah Bayegi, H Immunology Research Center - Avicenna Research Institute - Mashhad University of Medical Sciences, Mashhad , Goshayeshi, LE Microbiology and Virology Research Center - Avicenna Research Institute - Mashhad University of Medical Sciences, Mashhad , Amel Jamehdar, S Microbiology and Virology Research Center - Avicenna Research Institute - Mashhad University of Medical Sciences, Mashhad , Ghaffarzadegan, K Cancer Research Center - Omid Hospital - School of Medicine - Mashhad University of Medical Sciences, Mashhad
Abstract :
Background: Hepatitis C is a disease with significant global impact. The distribution of hepatitis C virus (HCV)
genotypes in Mashhad (the Northeast and the biggest city after the capital of Iran) is unknown. The purpose of this
study was to determine the prevalence of HCV genotypes among HCV seropositive patients, and to study the
relationship between types, virologic and demographic features of patients in Mashhad.
Methods: Three hundred and eighty-two clinical specimens obtained from HCV-infected patients referred to
Ghaem Hospital in Mashhad during a period of 2009 to 2010 were selected. HCV genotype was determined by
Nested PCR amplification of HCV core gene using genotype specific primers.
Results: Totally, 299 patients were male (79.9%). The most common HCV genotype was genotype 3a, with 150
(40%) of subjects. Genotype 1a was the other frequent genotype, with 147(39.2%) subjects. Frequency of genotypes
for 1b, 5 and 2 was 41(10.9%), 13(3.4%) and 9(2.4%), respectively. Mix genotype including 1a+1b in 4 (1.04%),
1a+3a in 3 (0.8%) was found in 7 patients. Four percent out of these samples had an undetermined genotype.
Among the hemophilia patient, there were 13(48.1%) genotypes as 1a, 3(11.1%) 1b and 10(37%) 3a, respectively.
Conclusion: The dominant HCV genotype among patients living in Mashhad was 3a. This study gives added
evidence of the predominant HCV genotypes in Iran.
Keywords :
HCV , Genotyping , Nested PCR , Iran
