Title of article :
Response of Melanomacrophage Centers in Yellowfin Seabream (Acanthopagrus latus) Immune Organs as an Immunohistological Biomarker in Short-term Exposure to Phenanthrene
Author/Authors :
Shirmohammadi, Mehrnaz Department of Marine Biology - Khorramshahr University of Marine Science and Technology, Khorramshahr , Ronagh, Mohammad Taghi Department of Marine Biology - Khorramshahr University of Marine Science and Technology, Khorramshahr , Salamat, Negin Department of Marine Biology - Khorramshahr University of Marine Science and Technology, Khorramshahr , Movahedinia, Abdolali Department of Marine Biology - Khorramshahr University of Marine Science and Technology, Khorramshahr , Hamidian, Gholamreza Department of Basic Sciences - University of Tabriz, Tabriz
Abstract :
Background: The aim of this study was to assess the changes in plasma levels of antioxidant
enzymes and melano-macrophage centers (MMCs) in immune tissues as biomarkers of yellowfin
seabream (Acanthopagrus latus) exposed to phenanthrene (Phe) for 14 days.
Methods: The research was carried out at Khorramshahr University of Marine Science and
Technology, Khorramshahr, Iran in Jan 2016. Thirty-six immature fish were intraperitoneally injected
with coconut oil (10 μl/g-bw) containing Phe (70mg/kg-bw) and compared with control group
(without injection). Then tissue and blood samples were obtained at 1, 4, 7 and 14 d after
injection.
Results: The SOD and CAT activity showed a significant decrease in fish exposed to Phe up to
day 7, then activity increased at day 14 in Phe-treated fish (P<0.05). The size and number of
MMCs in treated fish in spleen and head kidney were higher than control.
Conclusion: Changes in these parameters (SOD, CAT, and MMCs) might be used as useful
biomarkers for evaluating immunosuppressive Phe in fish.
Keywords :
Antioxidant Enzymes , Melano-Macrophage Centers , Phenanthrene , Yellowfin Seabream
Journal title :
Astroparticle Physics
