Author/Authors :
Hashemi, Ali Mahmoud Dental Implant Research Center - School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , Hashemi, Hamid Mahmoud Dental Implant Research Center - Dept. of Oral and Maxillofacial Surgery - School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , kahnamouii, shiva solahaye Craniomaxillofacial Research Center - Dept. of Oral and Maxillofacial Surgery, -School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , Mahmoud Hashemi, Talieh Dental Implant Research Center - School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , Agajani, Hassan Dept. of Cardiology - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Frozannia, Khalil Dept. of Cardiology - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Pournasrollah, Alireza School of Medicine - Tabriz University of Medical Sciences, Tabriz, Iran. , Sadeg, Rasoul School of Medicine - Tabriz University of Medical Sciences, Tabriz, Iran. , Estakhri, Rasoul Dept. of Pathology - School of Medicine - Tabriz University of Medical Sciences, Tabriz, Iran. , Razmpa, Ebrahim Dept. of Otolaryngology - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Bahrami, Naghmeh Dept. of Stem Cell - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran.
Abstract :
Statement of the Problem: Oral squamous cell carcinoma is the most common oral malignancy. Toll-like receptor (TLR) activation led to alterations in the levels of mRNA encoding the TLR accountable for recognizing the inducing agonist and cross-regulation of other TLR.
Purpose: The purpose of this study is determination of mitogen-associated protein kinase (MAPK) activation in human immortalized oral epithelial cell (HIOEC) line via up regulating of TLR7.
Materials and Method: expression of TLR7 was measured in HIOEC and normal cells by quantitative real-time polymerase chain reaction (qRT-PCR) and samples were calibrated by β-actin.
Results: Western blot analysis discovered high expression of TLR7 and MAPK in HIOEC cell lines. TLR7 was over-expressed in HIOEC cell line. Imiquimod-induced expression of interleukin (IL)-6, IL-8, and vascular endothelial growth factor (VEGF) was inhibited by TLR7 siRNA in HIOEC cells as determined by reverse transcription polymerase chain reaction (RT-PCR). Mean fluorescence intensity of nuclear p38 expression was determined in HIOEC cell lines (p< 0.05). RT-PCR analysis of IL-6, IL-8, and VEGF mRNA expression in HIOEC cells stimulated with imiquimod (1 μg/ml) for indicated time points.
Conclusion: TLR7 is functionally over-expressed in HIOEC cell line of oral squamous cell carcinoma and development of resistance to cisplatin in human oral squamous cell carcinoma might occur through the mechanism involving activation of TLR7 and its dependent signaling pathway.
