Author/Authors :
Qiao, Liman The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University - Wenzhou 325000 - Zhejiang, China , Hu, Xiaoxia Jinhua Central Hospital - Jinhua 321000 - Zhejiang, China , Lin, Yiping Jinhua Polytechnic - Jinhua 321007 - Zhejiang, China , Wei, Yanli Jinhua Polytechnic - Jinhua 321007 - Zhejiang, China , Wu, Meiling Jinhua Polytechnic - Jinhua 321007 - Zhejiang, China , Yao, Jingchan Jinhua Polytechnic - Jinhua 321007 - Zhejiang, China , Ying, Xiaoqian Jinhua Polytechnic - Jinhua 321007 - Zhejiang, China , Fu, Xiaoyan Jinhua Polytechnic - Jinhua 321007 - Zhejiang, China , Ding, Mingxing Jinhua Polytechnic - Jinhua 321007 - Zhejiang, China
Abstract :
In this study, a cocktail of probe drugs was used to assess whether lentinan could influence
the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo.
Materials and Methods: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral
dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam.
Then, we obtained blood in specific durations for the determination of plasma concentration of the probe
drugs using UPLC-MS/MS. We also evaluated the pharmacokinetic parameters using the DAS 2.0 software.
Results: We found that various concentrations of lentinan increased the activity of rat CYP1A2,
CYP3A4, CYP2D6, and CYP2C19 but not CYP2C9.
Conclusion: These findings suggest that clinical application of lentinan combination with CYP3A4,
CYP1A2, CYP2C19, or CYP2D6 should be given careful consideration as this may lead to herb-drug
interactions and hence treatment failure.
Keywords :
Cocktail , CYP , Herb-drug interaction , Lentinan , Probe drug
