Title of article
Presence of exoU and exoS Genes in Pseudomonas aeruginosa Isolated from Urinary Tract Infections
Author/Authors
Firouzi-Dalvand, Leila Department of Microbiology - North Tehran Branch, Islamic Azad University, Tehran, IR Iran , Pooladi, Mehdi Department of Biology - School of Basic Sciences - Science and Research Branch, Islamic Azad University, Tehran, IR Iran , Nowroozi, Jamileh Department of Microbiology - North Tehran Branch, Islamic Azad University, Tehran, IR Iran , Akhvan-Sepahi, Abbas Department of Microbiology - North Tehran Branch, Islamic Azad University, Tehran, IR Iran , Hooshiyar, Mansooreh Department of Chemistry - Applied and Scientific University, Pasargad, IR Iran
Pages
4
From page
8
To page
11
Abstract
Background: Pseudomonas aeruginosa is considered an opportunistic pathogen; several reports indicate that the organism can also cause infections in healthy hosts. Four effector proteins have been described in P. aeruginosa: exoU, exoS, exoT, and exoY. These genes that are translated into protein products related to type III secretion systems.
Materials and Methods: A total of 134 samples were isolated, and P. aeruginosa was identified using biochemical tests. Bacterial genomic DNA was extracted, and the presence of the exoS and exoU genes were detected by PCR. Biofilms were formed by culturing P. aeruginosaon glass slides in rich medium.
Results: The exoU (73%), exoS (62%) genes were detected from infections caused by P. aeruginosa in urinary tract infection patients. Among the 119 strains isolated from patients with urinary tract infections.
Conclusion: An improved understanding of virulence genes and biofilm formation in P.aeruginosa may facilitate the future development of novel vaccines and drug treatments.
Keywords
Pseudomonas aeruginosa , exoU , exoS , Urinary tract infection , Biofilm
Journal title
Astroparticle Physics
Serial Year
2016
Record number
2442421
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