Title of article :
Helicobacter Pylori in Children: Molecular Characterization, Antibiotics Resistance, and MLST of Isolated Strains in an Algerian Hospital
Author/Authors :
Raaf, Naïma Department of Microbiology - Faculty of Nature and Life Sciences - University Ferhat Abbas, Setif, Algeria , Amhis, Wahiba Central Laboratory of Clinical Biology - Ibn Ziri Bologhine Hospital, Algiers, Algeria , Benhassine, Fadhila Ibn Ziri Bologhine Hospital, Algiers, Algeria , Baiod-Chorfi, Safa Ibn Ziri Bologhine Hospital, Algiers, Algeria , Ouar-Korichi, Mounira Laboratory of Enterobacteriaceae and Related Bacteria - Institut Pasteur, Algiers Algeria
Abstract :
Background: Helicobacter pylori infection is generally acquired in childhood. Algeria is a country with a high prevalence of H. pylori infection. The aim of this work was to take stock of H. pylori infection in Algerian children.
Materials and Methods: About 31antral biopsies were cultured, and then antibiotic susceptibility testing was performed. The statuses of cagPAI and vacA s, m, I, and d regions were determined as well as geographical typing was done by Multi Locus Sequence Typing (MLST) method.
Results: Culture was H. pylori positive in 12 children. Only one resistance to clarithromycin and one to metronidazole were detected. Four out of six strains possessed cagPAI, and five out of six strains were identified as vacA s2m2i2d2. The five strains tested by MLST were of the hpEurope type.
Conclusion: This study revealed high prevalence of H. pylori infection and low resistance to antibiotics and reported for the first time in Algeria a genetic typing of H. pylori strains isolated from Pediatrics.
Keywords :
vacA , cagPAI , Antibiotics resistance , Gastric biopsies , Culture
