Title of article :
Abnormal hippocampal miR-1b expression is ameliorated by regular treadmill exercise in the sleep-deprived female rats
Author/Authors :
Mohammadipoor-ghasemabad, Lily Department of Biology - Faculty of Science - University of Sistan and Baluchestan - Zahedan, Iran , Sangtarash, Mohammad Hossein Department of Biology - Faculty of Science - University of Sistan and Baluchestan - Zahedan, Iran , Esmaeili-Mahani, Saeed Department of Biology - Faculty of Science - Shahid Bahonar University of Kerman - Kerman, Iran , Sasan, Hosein Ali Department of Biology - Faculty of Science - Shahid Bahonar University of Kerman - Kerman, Iran , Sheibani, Vahid Laboratory of Molecular Neuroscience - Neuroscience Research Center - Institute of Neuropharmacology - Kerman University of Medical Science - Kerman, Iran
Abstract :
The protective effect of regular running on sleep deprivation (SD)-induced cognitive
impairment has been revealed. In this study, we focused on the effects of regular exercise, sleep
deprivation and both of them together on the microRNA-1b (miR-1b) expression and their relation to
the behavioral parameters and brain-derived neurotrophic factor (BDNF) expression.
Materials and Methods: We used ovariectomized (OVX) female rats. The exercise program was mildmoderate
treadmill training for 4 weeks. 72 hr SD was achieved using the multiple platform method and the
spatial learning and memory parameters have been evaluated by the Morris water maze (MWM) test. The
levels of studied genes were quantified by real-time PCR.
Results: SD down-regulated pri-miR-1b, miR-1b (P˂0.05), and BDNF mRNA (P˂0.01) in the
hippocampus. Furthermore, female rats under exercise conditions showed significant up-regulation
of the miR-1b and BDNF mRNA (P˂0.001). In addition, miR-1b positively correlated with cognitive
function (P˂0.05) and BDNF mRNA (P˂0.01).
Conclusion: Our data demonstrated that regular treadmill exercise could reverse the down-regulation
of hippocampal miR-1b, which has a probable role in the SD-induced cognitive impairment.
Keywords :
Female rats , Hippocampus , Mir-1b , Sleep deprivation , Treadmill training
Journal title :
Astroparticle Physics
