Author/Authors :
Mohamadnia, Abdolreza Department of Biotechnology - School of Advanced Technologies in Medicine - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Azimi, Seyedeh Afrooz Department of Modern Sciences and Technologies - Faculty of Medicine - Mashhad University of Medical Sciences - Mashhad, Iran , Pasdar, Alireza Department of Modern Sciences and Technologies - Faculty of Medicine - Mashhad University of Medical Sciences - Mashhad, Iran , Sadegh Nia, Hamid Reza Division of Neurocognitive Sciences - Psychiatry and Behavioral Sciences Research Center - Mashhad University of Medical Sciences - Mashhad, Iran , Mosavi Jarrahi, Alireza Department Social Medicine - The Cancer Institute Research Center - Medical School - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Jamaati , Hamid Reza Shahid Beheshti University of Medical Sciences - Tehran, Iran , Kazempour Dizaji, Mehdi Shahid Beheshti University of Medical Sciences - Tehran, Iran , Dargahi, Hossein Management Sciences and Health Economic Department - Health Information Management Research Center - School of Public Health - Tehran University of Medical Sciences - Tehran, Iran , Bahrami, Naghmeh Tehran University of Medical Sciences - Tehran, Iran , Khosravi, Adnan Tobacco Prevention and Control Research Center - National Research Institute of Tuberculosis and Lung Diseases - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Bahrami, Naeim Shahid Beheshti University of Medical Sciences - Tehran, Iran , Daghighi, Shadi Shahid Beheshti University of Medical Sciences - Tehran, Iran
Abstract :
Background: Lung cancer is the leading cause of death, related to cancer in both men and women. MicroRNAs (miRNAs) play
key roles in regulating the genes that are responsible for apoptosis, and regulation of gene expression. MicroRNA expression is
deregulated in cancer. Many efforts have been made to detect cancer biomarkers. Extracellular miRNAs, which are directly released
from tumors, can be detected in circulating blood.
Objectives: The aim of this study was to examine the expression of miRNA-205 and -21 in lung cancer patients relative to healthy
controls.
Methods: This cross sectional study was performed in 50 healthy blood sample controls and 50 non-small cell lung cancer (NSCLC)
blood samples. The expression of two miRNAs (miR-21 and miR-205) in peripheral blood mononuclear cells (PBMCs) was evaluated,
using real-time polymerase chain reaction (real-time PCR) techniques. Statistical tests were carried out via the Mann-Whitney test.
Results: The miR-205 levels of expression in the patients with lung cancer significantly increased in comparison to healthy controls
(6.8 0.42 and 1.2 0.19, respectively), (P = 0.014). The expression of miR-21 in lung cancer (5.2 0.52) significantly increased
compared to the control group (1.60.14) (P < 0.001).
Conclusions: Based on our findings, lung cancer can enhance the ectopic expression of several miRNAs, which, in turn, play key
roles in impaired apoptosis and further cancerous progression.
Keywords :
Non-Small Cell Lung Cancer , miR-205 , miR-21 , Expression
