Title of article :
Beta Globin Frameworks in Thalassemia Major Patients from North Iran
Author/Authors :
Akhavan-Niaki, Haleh Genetic Laboratory - Amirkola Children's Hospital - Babol University of Medical Sciences , Banihashemi, Ali Genetic Laboratory - Amirkola Children's Hospital - Babol University of Medical Sciences , Azizi, Mandana Genetic Laboratory - Amirkola Children's Hospital - Babol University of Medical Sciences
Abstract :
Objective
Four combinations of five neutral sequence changes at rs713040, rs10768683, rs7480526, rs7946748, and rs1609812 occurring in the human beta globin gene defined as frameworks have been reported in beta globin gene. Here we report for the frequency of these frameworks in thalassemia major patients of North Iran.
Methods
Beta globin gene frameworks of 46 thalassemia major patients of North Iran were determined using Denaturing Gradient Gel Electrophoresis.
Findings
All these frameworks called framework 1, 2, 3, 3a were present at the frequency of 23.9%, 45.7%, 6.5% and 23.9% respectively.
Conclusion
These frameworks may be used for tracking mutant alleles in prenatal diagnosis programs.
Keywords :
Beta–Globin , Thalassemia , Polymorphism , Denaturing Gradient Gel Electrophoresis
Journal title :
Astroparticle Physics
