Title of article :
Differentiation Induction Effect of Mir-429 Over-Expression in U251 Glioma Cell Line
Author/Authors :
Gheidari, Fatemeh Department of Biotechnology - University of Tehran, Tehran , Arefian, Ehsan School of Biology - College of Science - University of Tehran, Tehran , Jamshidi-Adeghani, Fatemeh , Soleimani, Masoud Department of Hematology - Faculty of Medical Science - Tarbiat Modares University, Tehran
Abstract :
The purpose of this research was to investigate the effect of mir-429 ectopic over-expression
on U251 glioblastoma cell line. It has been shown that mir-429 could target BMK1 and reduce
glioma cells invasion. We cultured U251-mir-429 cells and observed changes in their morphology
for 14 days. They seemed to become wider with expanded cytoplasm and go on differentiation.
Neuronal (NEFM, NSE and neurogenin) and oligodendrocyte (MBP, OLIG2 and NKX2-2) gene
markers expression level was examined with real-time PCR. NSE, neurogenin, MBP, OLIG2
and NKX2-2 were significantly over-expressed, giving an evidence of cellular differentiation
in glioma cells.
Keywords :
Glioma , Mir-429 , BMK1 , Invasion
Journal title :
Astroparticle Physics
