Title of article
Method validation of methotrexate in human plasma by LC-MS technique in patients with brain tumour
Author/Authors
Danafar, Hossein Zanjan Pharmaceutical Nanotechnology Research Center - Zanjan University of Medical Sciences, Zanjan - Medicinal Chemistry Dept - Zanjan University of Medical Sciences, Zanjan - Pharmaceutics Dept - Zanjan University of Medical Sciences
Pages
10
From page
162
To page
171
Abstract
Background and aims: A selective and sensitive high performance liquid
chromatography-electrospray ionization mass spectrometry method has been established
for determination of methotrexate in human plasma.
Methods: Methotrexate was extracted from plasma with acetonitrile. The mobile phase
consisted of acetonitrile-water-formic acid 74: 25: 1(v/v). Twenty μl of sample was
chromatographically analyzed using a repacked ZORBAX-XDB-ODS C18 column
(2.1mm×30mm, 3.5 micron). The mode of mass spectrometry was selected-ion
monitoring (SIM).
Results: Standard curve was linear (r= 0.998) over the concentration range of 0.1–100.0
ng/ml and showed a suitable accuracy and precision. The limit of detection (LOD) was
0.05ng/ml.
Conclusion: The method is quick, easy, very steady and precise for the partition,
assignment and evaluation of methotrexate in human plasma.
Keywords
Methotrexate , LC-MS , Human plasma
Journal title
Astroparticle Physics
Serial Year
2016
Record number
2444430
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