Author/Authors :
Pereira Matsuchita, Hugo Leonardo Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil , de Faveri Pitz, Amanda Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil , Nesello Melanda, Francine Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil , Mitsuka Bregano, Regina Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil , Abreu de Oliveira, Francisco Jose Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil , Ruiz Lopes Mori, Fabiana Maria Philadelphia University Center - Londrina, Brazil , Claudia Noronha Dutra de Menezes, Maria Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil , Nazareth Costa, Idessania Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil , Rogerio Pavanelli, Wander Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil , Lucia Gomes, Monica Basic Health Sciences Dept - State University of Maringa - Maringa, Brazil , Colli, Cristiane Maria Basic Health Sciences Dept - State University of Maringa - Maringa, Brazil , Jose Venancio, Emerson Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil , Conchon-Costa, Ivete Pathological Sciences Dept - State University of Londrina - Rod - Londrina, Brazil
Abstract :
Background and aims: We investigated the children of Parana State, Brazil the prevalence of intestinal parasitosis and the associated factors involved in the transmission of intestinal parasites, and we genotyped the Giardia duodenalis isolates obtained.
Methods: Fecal samples were analyzed by established microscopic methods. G. duodenalis positive samples were subjected to genotypic characterization by PCR amplification of sequences of the glutamate dehydrogenase gene (gdh) and by enzymatic digestion with the restriction enzyme NlaIV for classification of genotypes.
Results: Of the 877 samples tested, 41% were positive for some intestinal parasitosis, the most common being the presence of protozoa (87.8%). Lack of basic sanitation and poor health education were associated for the intestinal parasite cases found, and the only associated factor for giardiasis was low family income. The G. duodenalis assemblages of gdh amplified samples were 68.6% B and 31.4% AII.
Conclusion: These data demonstrate the importance of epidemiological studies for the development of effective strategies with the aim of decreasing the incidence of intestinal parasites in children. Moreover, these results contribute to our knowledge of G. duodenalis assemblages circulating in the world and also offer support for future work on the molecular and clinical aspects of giardiasis.
