Bacteriological Evaluation of Aloe vera L. Fresh Gel on Experimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in Dogs

Objective - to evaluate both in-vivo and in-vitro antibacterial property of Aloe vera.L fresh gel. Design - experimental study. Animals - five adult male mixed breed dogs aged 2-4 years. Procedures - for in-vivo experiment, under general anesthesia and sterile condition 8 symmetrical full-thickness wounds measured 2 cm×2 cm were surgically created on the back of each dogs on 0, 7, 13 and 21 days of study. After wound creation, 1 ml fluid contains 105 CFU Staphylococcus aureus was inoculated on each wound. Right wounds in all dogs were covered with 1 ml Aloe vera fresh gel whereas the left wounds were not received any treatment. Antibacterial activity of Aloe vera gel was evaluated on days 7, 15, 21 and 28 by wound biopsies. Antimicrobial susceptibility test was applied for invitro evaluation. For this purpose, measurement of inhibition zone of Aloe vera gel was compared to some commercially available antibiotic discs. Results - no significant differences were seen by counting viable bacteria between the treatment and control wounds at the particular days of the period study (P > 0.05). The gel inhibited the growth of S.aureus by appearance of 12 mm inhibitory zone Conclusions and Clinical Relevance - Aloe vera has antibacterial property against S.areus.