Author/Authors :
Mohammadi-Roushandeh, A Department of Anatomy - School of Medicine - Tehran University of Medical Sciences , Noori-Mooghahi, M. H Department of Anatomy - School of Medicine - Tehran University of Medical Sciences , Pasbakhsh, P Department of Anatomy - School of Medicine - Tehran University of Medical Sciences , Abddvahabi, A Department of Anatomy - School of Medicine - Tehran University of Medical Sciences , Akbari, M Department of Anatomy - School of Medicine - Tehran University of Medical Sciences , Shokrgozar, A National Cell Bank of Iran (NCBI) - Pasteur Institute of Iran, Tehran , Sobhani, A Department of Anatomy - School of Medicine - Tehran University of Medical Sciences
Abstract :
Rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive
techniques. In this study we investigated the effects of supplementation of cysteamine on the rate of in
vitro maturation of oocytes in two different media. Germinal vesicle oocytes were collected from mouse
ovary and cultured in two media (TCM199 and MEME) with 0, 50, 100, 200, 500 μM/ml cysteamine.
Number of germinal vesicle breakdowns and metaphase II oocytes were recorded. The results showed
that the rate of in vitro maturation in 100 μM/ml cysteamine was significantly higher compared to
control (P < 0.05). Evaluation of two media in this study showed that TCM199 improved the rate of in
vitro maturation and oocyte maturation better than MEME; however, this difference was not statistically
significant. These findings indicate that TCM199 as compared to MEME was better in rate of in vitro
maturation of oocytes.
Keywords :
In vitro maturation of oocyte , cysteamine , glutathione , mouse
