Author/Authors :
Oveisi, M. R Department of Drug and Food Control - Faculty of Pharmacy - Tehran University of Medical Sciences , Sadeghi, N Department of Drug and Food Control - Faculty of Pharmacy - Tehran University of Medical Sciences , Hajimahmoodi, M Department of Drug and Food Control - Faculty of Pharmacy - Tehran University of Medical Sciences , Jannat, B Ministry of Health and Medical Education, Tehran , Behfar, A Department of Bromatology - Faculty of Pharmacy - Ahwaz University of Medical Sciences , Sobhani, H Department of Drug and Food Control - Faculty of Pharmacy - Tehran University of Medical Sciences
Abstract :
Fatty acids play important roles in biological systems and the newborns fatty acids
requirements are covered only by the milk. It is of particular interest to qualify the content of the fatty
acids in the milk. This study was performed to determine the levels of some fatty acids in the infant
formulas and also to describe a method without derivatization for the fatty acids analysis and applying it
to the control of infant formulas. Free fatty acids were produced by adding isopropanol- KOH to milk
fat extract and heating it to saponify and acidify by H2SO4. Free fatty acids were extracted and were
quantified by capillary gas chromatography on a fused silica column (AT-1000) and flame ionization
detector. The average experimental values of lauric, palmitic, stearic and linoleic fatty acids contents of
twenty infant formulas were 6.47, 16.52, 2.11 and 14.56 g/100g, respectively. The obtained
experimental values of lauric and linoleic fatty acids contents of twenty infant formulas were in good
agreement with the values proposed by standards of codex alimentary.
