Author/Authors :
Negahdari, Babak Dept. of Medical Biotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences , Shirazi, Mohamad Hasan Dept. of Pathobiology - School of Public Health - Tehran University of Medical Science , Vaise Malekshahi, Ziba Dept. of Medical Biotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences , Kadkhodazadeh, Maryam Dept. of Clinical Biochemistry - ShaheedBeheshti University of Medical Sciences , Hajikhani, Sara Dept. of Pathobiology - School of Public Health - Tehran University of Medical Science , Rahmati, Majid Dept. of Medical Biotechnology - School of Medicine - Shahroud University of Medical Sciences
Abstract :
Background: Campylobacter jejune and C. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. In this study Identification of Campylobacter Jejuni and Campylobacter Coli from samples using PCR was explored. Methods: Detection was performed using diarrheal samples collected 8 from 117 children.The genomic DNA of samples was extracted by phenol-chloroform method. All DNA extracts were examined for the presence of C.jejuni and C.coli species based on PCR method. Results: Of 117 diarrheal samples, 35 (29.9%) were found positive for 10 Campylobacter spp using PCR. Conclusions: The results of this study showed that PCR is effective for rapidly screening stool samples for Campylobacter spp, due to its high sensitivity and specificity.
Keywords :
Campylobacter jejuni , Campylobacter coli , Isolation , PCR
