Author/Authors :
Abbasi, N Department of Medical Biochemistry - School of Medicine - Medical Sciences University of Tehran , Khaghani, S Department of Medical Biochemistry - School of Medicine - Medical Sciences University of Tehran , Sharif-Tabrizi, A Department of Medical Biochemistry - School of Medicine - Medical Sciences University of Tehran , Farzami, B Department of Medical Biochemistry - School of Medicine - Medical Sciences University of Tehran , Vardasbi, S Department of Medical Biochemistry - School of Medicine - Medical Sciences University of Tehran , Bahar,, M Department of Medical Biochemistry - School of Medicine - Medical Sciences University of Tehran , Ansari, M Department of Medical Biochemistry - School of Medicine - Medical Sciences University of Tehran , Khorramizadeh, R Department of Medical Biochemistry - School of Medicine - Medical Sciences University of Tehran , Gerayesh-Nejad, S Department of Medical Biochemistry - School of Medicine - Medical Sciences University of Tehran
Abstract :
To determine the importance of Q10H2 as an antioxidant in cancer, we measured the activity
of lipoamide dehydrogenase (Q10H2 recycling enzyme) in HL-60 and normal lymphocyte. The cultured
cells of HL-60 and human normal lymphocytes were assayed in cell lysate of given number of both HL-
60 and normal lymphocyte. The activity of lipoamide dehydrogenase and the protein concentration were
determined by spectrometric methods. The activity of LAD was found to be 0.216μmol/min/μg protein
in HL-60 cells and 0.0415μmol/min/μg protein in normal lymphocytes. Although the average protein
concentration ratio in HL-60 cells to normal lymphocyte was found to be %124, the average ratio of
enzyme activity in HL-60 to normal lymphocyte was %526. These results are indicative of
independence of enzyme increase in HL-60 cells of increase of protein synthesis.
Keywords :
Lipoamide dehydrogenase , Lymphocyte , HL-60 , Leukemia
