Author/Authors :
Rafati, Mohammad Colorectal Research Center, Shiraz University of Medical Sciences , Hosseini, Vahid Colorectal Research Center, Shiraz University of Medical Sciences , Zamani, Mozhdeh Colorectal Research Center, Shiraz University of Medical Sciences , Khazraei, Hajar Colorectal Research Center, Shiraz University of Medical Sciences , Moradian, Farid Department of Surgery, Yasouj University of Medical Sciences , Mokhtari, Maral Department of Pathology, Shiraz University of Medical Sciences
Abstract :
Background: Perianal fistula is a complicated disorder and
most difficult to manage. New treatment methods would help
surgeons to achieve a better outcome in patients with perianal
fistula. Human amniotic membrane (HAM) has positive effects
on wound healing in several conditions. The present study aimed
to further determine the effect of HAM on wound healing of
perianal fistula in rabbits.
Methods: In a prospective experimental study, 14 male rabbits
(aged 4-6 months and weighing 3-4 kg) were randomly divided into
2 groups. After 12 weeks, the high type perianal fistula was repaired
with endorectal flap (ERF) and ERF plus HAM in the control and
case groups, respectively. In all rabbits of the case group, a 1×1 cm
width wrap of HAM was applied and fixed around the ERF site.
Three weeks later, the repaired site of the perianal fistula was sent
for pathologic wound healing scoring. The results were analyzed
with the SPSS 21.0 software using Mann-Whitney test.
Results: Six rabbits of each group survived the study period.
There was a statistically significant difference in wound healing
between the case and control groups (P<0.001). Wound healing
process in the case group occurred better and faster than the
control group.
Conclusion: HAM has an effective role in enhancing the ERF
procedure and considered appropriate. A combination of HAM
with other methods is recommended.
