Author/Authors :
Poursafa, Parinaz Environmental Protection Engineer, Department of Environment and Energy - Science and Research Branch - Islamic Azad University, Tehran , Kelishadi, Roya Professor of Pediatrics - Pediatrics Department - Child Health Promotion Research Center, Isfahan University of Medical Sciences , Moattar, Faramarz Professor - Faculty of Environment and Energy, Science and Research Branch - Islamic Azad University, Tehran , Rafiee, Laleh Research Assistant - Department of Physiology - Applied Physiology Research Center, Isfahan University of Medical Sciences , Amin, Mohammad Mehdi Associate Professor of Environmental Health Engineering - Department of Environmental Health Engineering - Environment Research Center, Isfahan University of Medical Sciences , Lahijanzadeh, Ahmadreza Isfahan Provincial Directorate of Environmental Protection , Haghjooy Javanmard, Shaghayegh Assistant Professor of Physiology - Department of Physiology - Applied Physiology Research Center, Isfahan University of Medical Sciences
Abstract :
BACKGROUND: Some experimental studies revealed that exposure to air pollution increases the expression of tissue factor
(TF) in atherosclerotic lesions. We aimed to investigate the role of TF +5466A>G (rs3917643) polymorphism in the
association of air pollution on serum levels of TF as a biomarker of vascular injury in children.
METHODS: This cross-sectional study was conducted among 110 children, consisting of 58 (52.8%) girls and 52
(47.2%) boys with a mean age of 12.7 + 2.3 years, living in Isfahan, Iran. Enzyme-linked immunosorbent assay were
used for measurement of serum TF. Genotype of +5466A>G (rs3917643) polymorphism was determined by the polymerase
chain reaction–restriction length fragment polymorphism (PCR–RFLP) method.
RESULTS: We identified 2 individuals with +5466AG genotype and 108 homozygous for the +5466A allele (no
+5466GG homozygotes). The mean pollution standards index (PSI) value was at moderate level, the mean particular
matter measuring up to 10 μm (PM10) was more than twice the normal level. Multiple linear regression analysis showed
that after adjustment for confounding factors (weight status, dietary and physical activity pattern), serum TF level had
significant relationship with PSI (beta: 0.55, SE: 0.07, p<0.000) and PM10 (beta: 0.51, SE: 0.03, p=0.001).
CONCLUSIONS: In spite of similar genetic polymorphism of TF, air pollutants might have an independent association
with systemic inflammatory and coagulation responses. The harmful effects of air pollutants on the first stages of atherosclerosis
in the pediatric age group should be underscored in primordial and primary prevention of chronic diseases
Keywords :
Atherosclerosis , Prevention , Air pollution , Genetics , Children
