Effect of Sprayed Cultured Autologous Keratinocyte Suspension Used Alone and in Combination with Fibrin Glue to Closure of 3rd Degree Burn Wounds in Rat

Objective- Our point is to determine if the treatment of the 3rd degree burn wounds makes any difference if sprayed cultured autologous keratinocyte used alone or in combination with Fibrin glue. Design- Animal experimental study. Animals- 30 male Wistar rats. Procedures- Thirty male Wistar rats were selected and their keratinocytes were isolated and cultured from small skin biopsy. Rats were divided randomly into 3 equal groups and three 3rd degree burn wounds with 1 cm diameter were created on their back. Wounds were treated with normal saline (control group), Autologous keratinocyte suspension (AKS) (test group1) and AKS + Fibrin (AKS+ F) (test group2) in each rat. The wounds were photographed on selected days (0, 3, 5, 7, 10 and 14) and the percentage of wounds contraction was calculated with image analyzer. Biopsy samples were taken as well for histological studies on days 3, 7 and 14. Results-The results showed faster wound contraction for AKS and AKS+ F groups during 14 days period than control group (P<0.05). Histological observations showed significant difference in inflammation and fibrotic tissue formation between groups but other parameters did not show any remarkable difference. Conclusion and Clinical Relevance- Although it can be concluded that fibrin glue could prevent cells from dripping out of the wound and also speed up the wound contraction and extend the inflammation and fibrotic tissue formation, it did not have any effect on fastening the re-epithelialization and granulation tissue formation during 14 days.