Author/Authors :
Dehghani, Leila Department of Medical Sciences, Islamic Azad University, Najafabad Branch , Poorazizi, Elahe Department of Medical Sciences, Islamic Azad University, Najafabad Branch , Meamar, Rokhsareh Department of Medical Sciences, Islamic Azad University, Najafabad Branch , Hashemi-Beni, Batool Department of Anatomical Sciences, Isfahan University of Medical Sciences , Khorvash, Fariborz Isfahan Neurosciences Research Centre, Isfahan University of Medical Sciences , Shaygannejad, Vahid Isfahan Neurosciences Research Centre, Isfahan University of Medical Sciences , Vesal, Sahar Isfahan Neurosciences Research Centre, Isfahan University of Medical Sciences , Sedghi, Maryam Genetics Laboratory - Alzahra University Hospital, Isfahan University of Medical Sciences, Isfahan
Abstract :
Previous studies confirmed that neural gene expression in embryonic stem cells (ESC) could influence by chemical
compounds through stimulating apoptotic pathway. We aimed to use ESCs‑derived neural cells by embryoid body formation as an
in vitro model for determination of neural gene expression changes in groups that treated by sera from Alzheimer’s patients and
compare with healthy individuals. Materials and Methods: ESC line which was derived from the C57BL/6 mouse strain was used
throughout this study. ESC‑derived neural cells were treated with serum from Alzheimer’s patient and healthy individual. Neural gene
expression was assessed in both groups by quantitative real‑time polymerase chain reaction analysis. The data was analyzed by SPSS
Software (version 18). Results: Morphologically, the reducing in neurite out‑growth was observed in neural cells in group, which
treated by serum from Alzheimer’s patient, while neurite growth was natural in appearance in control group. Microtubule‑associated
protein 2 and glial fibrillary acidic protein expression significantly reduced in the Alzheimer’s patient group compared with the control
group. Nestin expression did not significantly differ among the groups. Conclusion: Neural gene expression could be reduced in
serum treated ESC in Alzheimer’s patients.
